Next-Generation Sequencing Solutions for Lymphoid Genomic Profiling

The need for more comprehensive lymphoid testing is growing

As the list of known mutations and relevant genes for lymphoid disorders increases, characterizing these samples becomes more complicated and time-consuming.

To get meaningful genetic insights, you need a rapid and efficient approach for evaluating all key mutations upfront.

Next-generation sequencing (NGS) can streamline the path from sample to result by providing a comprehensive molecular profile with speed
and simplicity.

lymphoid-ngs-header

krigstein

Presenter
Dr. Michael Krigstein
Consultant Haematologist
MBBS(Hons) FRACP FRCPA MCncrSc
SydPath, St Vincent’s Hospital
Sydney, Australia

On-demand Webinar

Utility of NGS in Lymphoid Malignancies and Ion AmpliSeq™ Liverpool Lymphoid Network Panel experience

NGS has become a key component of molecular testing in both myeloid and lymphoproliferative neoplasms. It can facilitate improvements in classification, prognostication and identification of therapeutic targets. In this presentation, hear our expert share the utility of faster molecular results in lymphoid genomic profiling and their experience with the Ion Torrent Genexus System and the Ion AmpliSeq™ Liverpool Lymphoid Network Panel.

Explore lymphoid NGS solutions

Ion AmpliSeq Liverpool Lymphoid Network Panel

Oncomine Lymphoma Panel

Ion AmpliSeq Liverpool Lymphoid Network Panel

The Ion AmpliSeq Liverpool Lymphoid Network Panel is a community panel designed by leading clinical researchers for more comprehensive lymphoid genomic profiling.

Key Features

Rapid	Automated	Comprehensive	Verified Performance	Community Network

Go from specimen to result in as little as one day 6	Process samples with minimal hands-on time, available on both GeneStudio & Genexus 7	Simultaneously profile 60 key DNA mutations relevant for a broad range of lymphoid disorders 29	Reliably detect variants using a panel designed and tested by community users with real-world clinical research samples 79	Access a community network of labs and contribute to future iterations of the panel design 99

Profile 60 Key DNA Mutations With One Test

Panel content has been curated to cover relevant targets for T-cell and B-cell lymphomas, leukemias and other lymphoproliferative disorders. Compatible with all common sample types, including blood, bone marrow and formalin-fixed paraffin embedded (FFPE) tissue.

Ion AmpliSeq Panel Design includes:

Essential exons and full coding sequence coverage of key genes
3’ UTR coverage of NOTCH1
Non-coding exon 1 of BCL6 and partial intron 1

DNA Panel (60 Genes)

Essential Exons (36)		Full Coding Sequence (24)
BCL6	IRF4	ARID1A	PAX5
BIRC3	KRAS	ATM	PIM1
BRAF	MAP2K1	B2M	PRDM1
BTK	MYD88	BCL2	PTEN
CARD11	NOTCH1	CDKN2A	SAMHD1
CCND1	NOTCH2	CREBBP	SGK1
CCND3	NRAS	DIS3	SOCS1
CD79B	PLCG2	GNA13	TENT5C
CXCR4	POT1	ID3	TET2
DNMT3A	RHOA	KMT2D	TNFAIP3
EP300	RPS15	MEF2B	TP53
ETV6	SF3B1	MYC
EZH2	SMARCA4	NFKBIE
FAS	STAT3
FBXW7	STAT5B
FOXO1	STAT6
HRAS	TNFRSF14
IDH2	XPO1

> Learn more about Community Panels

Oncomine Lymphoma Panel

The Oncomine Lymphoma Panel contains 25 key genes associated with all major B-cell lymphomas, including DLBCL, follicular lymphoma, mantle cell lymphoma, and marginal zone lymphomas.

This panel provides robust performance with as little as 10 ng of input and comes with an end-to-end, clinical research-grade workflow including straightforward, customizable report that summarizes the findings.

Available for Ion Chef and GeneStudio S5 Systems

Lymphoma
ARID1A	BRAF	CDKN2A	HIST1H1E	MYD88	TNFAIP3
ATM	BTK	CREBBP	KMT2D	PIM1	TNFRSF14
B2M	CARD11	EZH2	MTOR	SOCS1	TP53
BCL2	CD79B	GNA13	MYC	SF3B1	XPO1
BCL6

Customize the assay to suit your needs by selecting from a library of primer designs to
add additional gene targets.

> Learn more about Oncomine Tumor-Specific Panels

Explore our NGS Sequencers

Explore our next-generation sequencers and see which one is right for your lab.

Genexus System

The Ion Torrent Genexus System automates sample and library preparation, sequencing, analysis, and reporting.

> Explore Genexus

Ion GeneStudio S5 System

The Ion GeneStudio S5 systems are designed to enable a broad range of targeted next-generation sequencing applications with speed and scalability.

> Explore GeneStudio

Additional Resources

Oncomine Immune Repertoire Flyer

Oncomine Myeloid Assay Gx v2 Flyer

Get in Touch

Request a quote or personal demonstration from a Thermo Fisher Scientific Representative.

*For Research Use Only. Not for use in diagnostic procedures.

Case Number: 52969 (0424)

Next-Generation Sequencing Solutions for Lymphoid Genomic Profiling

The need for more comprehensive lymphoid testing is growing

Utility of NGS in Lymphoid Malignancies and Ion AmpliSeq™ Liverpool Lymphoid Network Panel experience

Explore lymphoid NGS solutions